Delivering bestofclass performance in all aspects the mivac dna provides scientists everything they need to safely and swiftly concentrate or dry their samples in a compact, easytouse benchtop unit. The dna 110s oilfree vacuum pump provides maintenancefree operation, and the unit is corrosion resistant for years of lasting service. Centrivap micro ir vacuum concentrators small, yet complete for small throughput and personal use, the centrivap micro ir vacuum concentrator has an infraredemitting lid to add heat to the chamber and reduce condensation. Smallvolume samples can be efficiently processed by this model. If low yields are a problem, a micro ir concentrator can very easily help you increase the yields in one easy step. Choose from the duo concentrator for low sample numbers or the quattro sample concentrator for larger numbers. Genevacs mivac dna concentrator improves forensic sample. This product facilitate the removal of dna polymerases, modifying enzymes, rna polymerases, ligases, kina.
Eluted dna is suitable for sequencing, pcr, endonuclease digestion, and other enzymatic procedures. This centrifugal concentrator is capable of removing low volumes of water and organic solvents from a variety of sample formats including tubes, microplates, vials, and round bottom flasks. The virus it produces is certainly able to transduce 293t cells very well. Unique column construction allows sample washing to be performed using a centrifuge or vacuum source. Mivac dna system the mivac dna integrated system is a centrifugal concentrator capable of removing water and organic solvents from a variety of sample formats and is designed specifically for working with nucleic acids. Scientific savant speedvac dna is a compact, integrated vacuum concentrator designed for fast, efficient concentration of dna and rna applications. Savant speedvac dna integrated vacuum concentrator.
Dec 10, 2009 ive tried the lentix concentrator twice now and it works reasonably well. Simply heating the solution in a driblock heater does not greatly increase the evaporation rate. The centrivap dna centrifugal concentrator is expressly designed to speed evaporation of solvents from dna and other very small samples. Centrivap centrifugal vacuum concentrators use a combination of centrifugal force, vacuum and heat to speed laboratory evaporation of multiple small samples. If the address matches an existing account you will receive an email with instructions to reset your password. Kinetics and dna replication if youre seeing this message, it means were having trouble loading external resources on our website. For purification of short dna or rna oligonucleotides. Centrivap centrifugal concentrators and cold traps labconco. Thermo savant dna 110 speedvac concentrator marshall scientific.
A04001b concentration of dna with sample concentrator. Has anyone tried using speedvac to concentrate dna samples. What is the optimum conditionsetting for the speedvac so that the dna wont be. Im working out my titre using flow cytometry on serial dilutions. We noticed several instances of observed contaminating. A04001b concentration of dna samples using the techne sample concentrator introduction the techne sample concentrator uses the principle of solvent evaporation to concentrate samples. Simply add the specially formulated dna binding buffer to the sample and transfer to the supplied column. Lentivirus concentrator solution dissolve 80g peg8000, 14. Making it an ideal concentrator for the busy molecular biology laboratory. This compact, benchtop cabinet contains all necessary components required to begin immediate concentration processing. User manual mivac modular concentrator series 045057 issue 26 september 2012 basic operation power up the duo, dna or quattro concentrator, powers up as soon as it is connected to mains power. Thermo savant dna 120 speedvac concentrator the thermo savant dna 120 speedvac concentrator offers 100% recovery for rapid and efficient concentration and drying of dna rna samples, all with quiet operation and a small footprint. Centrivap cold traps protect your vacuum pump from corrosive chemicals. The dna 120s oilfree vacuum pump provides maintenancefree operation, and the unit is corrosion resistant for.
As a successor of the dna 110 speedvac concentrator, dna 120 units are equipped with the. It has a small footprint yet is packed with features. The dna 120 speedvac concentrator is a robust system for technical dna rna drying needs. Getting dna samples ready for analysis can be the most time consuming and critical step for dna technicians in the forensic lab. The savant dna 110 speedvac concentrator offers 100% recovery for rapid and efficient concentration and drying of dna rna samples, all with quiet operation and a small footprint. Savant dna 110 speedvac concentrator marshall scientific. There is no need for organic denaturants, chloroform, or messy precipitations. Just right for plugandplay processing, these allinone concentrators house a vacuum concentrator, cold trap.
Effect of operating parameters on the performance of spiral concentrator results and discussion spiral is operated at three different sets of splitter positions and at three different flow rates ranging from low to high. Genevac mivac dna vacuumintegrated centrifugal concentrator system can remove water and organic solvents from a variety of sample formats. High quality, inhibitorfree dna is crucial to ensure success with qpcr. This video shows the dnav model for young people the thriving adolescent. Concentrated dna is ready for downstream application. High quality, inhibitorfree dna is crucial to ensure success with qpcr, dna ligationcloning, sequencing. Browse a full range of vacuum concentrators products from leading suppliers to meet your needs. Sartorius vivacon 500 dna concentrator life sciences. Specially designed centrifugal concentrator and integrated vacuum for working with nucleic acids. The mivac dna is capable of removing low volumes of water and organic solvents from a variety of sample formats including tubes, microplates, vials and round bottom flasks. All segments of the lcd screen temporarily switch on and illuminate to test they are functioning correctly.
A centrifugal concentrator capable of removing water and organic solvents from a variety of sample formats including tubes, microplates and vials. These experiments are carried for four feed pulp densities 25, 30, 35 and 40 wt%. The dna concentrator kit is a complete set of essential components which enables the experimenter to efficiently concentrate dna from various dna samples with low concentration of dna including those from microdissection samples and paraffinembedded tissues. The mivac dna from genevac is designed to safely and quickly evaporate solvents from sensitive dna, rna and other biological samples. Sep 20, 2016 genevac report on a paper by uk researchers describing use of its mivac dna concentrator to provide reliable and efficient sample preparation in the development of a novel forensic method to. Any suggestions on concentrating dna using speedvac. Thermo scientific savant speedvac dna is a compact, integrated vacuum concentrator designed for fast, efficient concentration of dna and rna applications. Ive used it on 3 different lentiviruses so far and have had 20x, 20x and 7x increase in virus titre much less than 100x. The dna concentrator kit simply applies our proprietary dna binding buffer to dna sample. The entire procedure can be completed within 2 minutes and is suitable for. Making it an ideal workhorse concentrator for the busy lab. After transferring the sample to the specially designed fastspin column and washing it with the washing buffer, the dna is easily recovered in 810 l with elution solution.
The mivac dna is an ideal small concentrator for the molecular biology laboratory. They are also the product of choice for protein fractionation applications in this volume range. Often in order to produce reliable results, the yield of the dna needs to be increased. If youre behind a web filter, please make sure that the domains. Eluted dna is ideal for pcr, dna sequencing, dna transfection, dna ligation, endonuclease. Speedvac dna vacuum concentrator will support sample preparations with ease and efficiency. Micro ir vacuum concentrator increases yields in dna analysis. Simply add the specially formulated dna binding buffer to your sample and transfer to the supplied zymospin column. The dna concentrator kit ab156895 is a complete set of essential components which enables the experimenter to efficiently concentrate dna from various dna samples with low concentration of dna including those from microdissection samples and paraffinembedded tissues. Vivacon 500 are centrifugal devices for dna concentration and buffer exchange applications for samples up to 500l. A centrifugal concentrator capable of removing water and organic solvents from a variety of sample formats. Effect of operating parameters on the performance of spiral. Eto treated vivacon concentrators for dna samples our laboratory has traditionally used untreated vivacon concentrators to perform cleanup and concentration of samples after extraction with stain extraction buffer and phenolchloroformisoamyl alcohol. Benchtop cabinet houses the concentrator, dna rotor, diaphragm, vacuum pump, two glass traps and all connecting tubing.
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